Food Science and Technology

ISSN-print: 2073-8684
ISSN-online: 2409-7004
ISO: 26324:2012
Архiви

ПЕРСПЕКТИВИ ВИКОРИСТАННЯ ГЛЮКОЗО-ФРУКТОЗНОГО СИРОПУ В БІОТЕХНОЛОГІЇ РИБОФЛАВІНУ

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V. Polishchuk
http://orcid.org/0000-0002-1284-584X
O. Dugan
http://orcid.org/0000-0001-5594-6088

Анотація

Riboflavin is an important vitamin widely used in the food industry to enrich food and as a colourant. An important problem in the implementation of the riboflavin biotechnology is selecting rational, i. e. low-cost and technologically simple sources of carbon and nitrogen. It can significantly increase the efficiency of this technology. Fungi of the genus Eremothecium are known to be capable of synthesising aromatic substances. Studying the level of essential oil accumulation in the carbon and nitrogen sources suggested will allow creating technologies for the simultaneous production of riboflavin and essential oil. The object of study was the ascomycete Eremothecium ashbyi Guillierm. F-340. The biosynthetic activity of the selected producer strain of riboflavin on media with different carbon and nitrogen sources has been studied, as well as the composition of a rational growth medium to achieve the maximum riboflavin accumulation when culturing the strain, and the producer’s ability to synthesise aromatic compounds on this medium. It has been shown how various carbon and nitrogen sources influence biomass accumulation and riboflavin synthesis by the strain E. ashbyi F-340. Monosaccharides (fructose, galactose) and hexatomic alcohol sorbitol have been found to be best suited for the maximum riboflavin accumulation. The best source of nitrogen was yeast extract. The data obtained prove the effectiveness of glucose-fructose syrup with the fructose content 8–12% on a dry basis (GFS-10). It has been shown that the largest vitamin amount (140 mg/dm3) is synthesised when using GFS-10. Wide-ranging variation in the content of the synthesised essential oil has been revealed, the most of it being observed in the medium containing GFS-10 (273–453 mg/dm3) as a carbon source. The use of GFS-10 has made it possible to increase the riboflavin yield by 6.7 times as compared to the medium with glucose, and by 3.7 times as compared to the medium with fructose. The yield of essential oil has increased by a factor of 5. The data obtained can be considered a prerequisite of optimising the nutrient medium

Ключові слова:
riboflavin, Eremothecium ashbyi, carbon and nitrogen sources, glucose-fructose syrup, essential oil

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Як цитувати
Polishchuk, V., & Dugan, O. (2020). ПЕРСПЕКТИВИ ВИКОРИСТАННЯ ГЛЮКОЗО-ФРУКТОЗНОГО СИРОПУ В БІОТЕХНОЛОГІЇ РИБОФЛАВІНУ. Food Science and Technology, 14(2). https://doi.org/10.15673/fst.v14i2.1512
Розділ
Біопроцеси, біотехнологія харчових продуктів, БАР